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anti trpa1  (Alomone Labs)


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    Structured Review

    Alomone Labs anti trpa1
    Anti Trpa1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trpa1/Anti-TRPA1+(extracellular)+Antibody/bio_rxiv__64898__2026__05__11__724406-42-19-23
    Average 95 stars, based on 55 article reviews
    anti trpa1 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Western Blot:

    Article Title: Schwann cell Lrp1 deletion drives trigeminal neuron sensitization and orofacial pain by modulating mitochondrial function and TRPV1/TRPA1 activity.
    Article Snippet: .. Primary antibodies and dilutions: MFN2 (Proteintech, #12186–1-AP, Rabbit, 1:1000); Phospho-PDH (pPDH) (Cell Signaling Technology, #31866, Rabbit, 1:1000); Total PDH (tPDH) (Cell Signaling Technology, #3205, Rabbit, 1:1000); Total OXPHOS Rodent WB Antibody Cocktail (Abcam, ab110413, Mouse, 1:1000); TRPV1 (Alomone Labs, Israel, ACC-030, Rabbit, 1:500); TRPA1 (Alomone Labs, Israel, ACC-037, Rabbit, 1:500). .. Secondary antibodies: Anti-Rabbit IgG, HRP-linked (Cell Signaling Technology, #7074, 1:3000); Anti-Mouse IgG, HRP-linked (Cell Signaling Technology, #7076, 1:3000).

    Article Title: Schwann cell Lrp1 deletion drives trigeminal neuron sensitization and orofacial pain by modulating mitochondrial function and TRPV1/TRPA1 activity
    Article Snippet: .. Primary antibodies and dilutions: MFN2 (Proteintech, #12186–1-AP, Rabbit, 1:1000); Phospho-PDH (pPDH) (Cell Signaling Technology, #31866, Rabbit, 1:1000); Total PDH (tPDH) (Cell Signaling Technology, #3205, Rabbit, 1:1000); Total OXPHOS Rodent WB Antibody Cocktail (Abcam, ab110413, Mouse, 1:1000); TRPV1 (Alomone Labs, Israel, ACC-030, Rabbit, 1:500); TRPA1 (Alomone Labs, Israel, ACC-037, Rabbit, 1:500). .. Secondary antibodies: Anti-Rabbit IgG, HRP-linked (Cell Signaling Technology, #7074, 1:3000); Anti-Mouse IgG, HRP-linked (Cell Signaling Technology, #7076, 1:3000).

    Activation Assay:

    Article Title: TRPA1 contributes to respiratory depression from tobacco aerosol.
    Article Snippet: Transient receptor potential ankyrin-1 (TRPA1) is expressed in the trigeminal nerves in the nasal cavity.. It detects irritant chemicals such as formalin and acrolein, induces respiratory depression to protect against further inhalation, and elicits avoidance behavior.. Although tobacco smoke contains formalin, acrolein, and other irritant chemicals, the possible contribution of TRPA1 to protection against tobacco smoke has yet to be fully understood.

    Aerosol:

    Article Title: TRPA1 contributes to respiratory depression from tobacco aerosol.
    Article Snippet: Transient receptor potential ankyrin-1 (TRPA1) is expressed in the trigeminal nerves in the nasal cavity.. It detects irritant chemicals such as formalin and acrolein, induces respiratory depression to protect against further inhalation, and elicits avoidance behavior.. Although tobacco smoke contains formalin, acrolein, and other irritant chemicals, the possible contribution of TRPA1 to protection against tobacco smoke has yet to be fully understood.

    Staining:

    Article Title: TRPA1 contributes to respiratory depression from tobacco aerosol.
    Article Snippet: Transient receptor potential ankyrin-1 (TRPA1) is expressed in the trigeminal nerves in the nasal cavity.. It detects irritant chemicals such as formalin and acrolein, induces respiratory depression to protect against further inhalation, and elicits avoidance behavior.. Although tobacco smoke contains formalin, acrolein, and other irritant chemicals, the possible contribution of TRPA1 to protection against tobacco smoke has yet to be fully understood.

    Article Title: Few-layered graphene increases the response of nociceptive neurons to irritant stimuli.
    Article Snippet: The fluorescently conjugated secondary antibodies were labeled by Alexa Fluor 488 or Alexa Fluor 568 (#11029 and #A11036, respectively; ThermoFisher Scientific, Molecular Probes). .. Finally, after another washing round, neurons were stained with Hoechst33342 diluted 1 : 100 in BSA (2%) for 5 min. For studies on the channel distribution, polyclonal antiTRPV1 (ACC-334, Alomone labs) and TRPA1 (ACC-037, Alomone labs) were used, and the permeabilization step with 0.1% Triton X-100 was not performed, in order to bind only to the extracellular domain. .. The stained coverslips were mounted in Vectashield (H-1000-10; Vector Laboratories, Newark, USA) on 1 mm thick microscope slides.

    Immunohistochemistry:

    Article Title: Harnessing theta-gamma coupled brainwaves using ultrasound for spinal astrocyte revitalization and sustained neuropathic pain relief in mice
    Article Snippet: The tissue was then sectioned at 30 μm into cold PBS using a vibratome (Leica VT1000S) and stored at 4 °C. .. For spinal cord immunohistochemistry, spinal sections were immunolabeled for GFAP, GABA, BDNF, Iba1 (1:1000, ab225260), NeuN (1:1000, Millipore, MAB377), c-Fos (1:1000, SYSY, 226-008), and TRPA1 (1:500, Alomone lab, ACC037; 1:250, Abcam, ab58844). ..

    Article Title: Harnessing theta-gamma coupled brainwaves using ultrasound for spinal astrocyte revitalization and sustained neuropathic pain relief in mice.
    Article Snippet: The tissue was then sectioned at 30 μm into cold PBS using vibratome Leica VT1000S and stored at 4°C. .. For spinal cord immunohistochemistry, spinal sections were immunolabeled for GFAP, GABA, BDNF, Iba1 (1:1000, ab225260), NeuN (1:1000, Millipore, MAB377), c-Fos (1:1000, SYSY, 226-008), TRPA1 (1:500, Alomone lab, ACC037; 1:250, Abcam, ab58844). ..

    Immunolabeling:

    Article Title: Harnessing theta-gamma coupled brainwaves using ultrasound for spinal astrocyte revitalization and sustained neuropathic pain relief in mice
    Article Snippet: The tissue was then sectioned at 30 μm into cold PBS using a vibratome (Leica VT1000S) and stored at 4 °C. .. For spinal cord immunohistochemistry, spinal sections were immunolabeled for GFAP, GABA, BDNF, Iba1 (1:1000, ab225260), NeuN (1:1000, Millipore, MAB377), c-Fos (1:1000, SYSY, 226-008), and TRPA1 (1:500, Alomone lab, ACC037; 1:250, Abcam, ab58844). ..

    Article Title: Harnessing theta-gamma coupled brainwaves using ultrasound for spinal astrocyte revitalization and sustained neuropathic pain relief in mice.
    Article Snippet: The tissue was then sectioned at 30 μm into cold PBS using vibratome Leica VT1000S and stored at 4°C. .. For spinal cord immunohistochemistry, spinal sections were immunolabeled for GFAP, GABA, BDNF, Iba1 (1:1000, ab225260), NeuN (1:1000, Millipore, MAB377), c-Fos (1:1000, SYSY, 226-008), TRPA1 (1:500, Alomone lab, ACC037; 1:250, Abcam, ab58844). ..

    Blocking Assay:

    Article Title: Schwann cell transient receptor potential ankyrin 1 (TRPA1) ortholog in zebrafish larvae mediates chemotherapy-induced peripheral neuropathy.
    Article Snippet: .. The slides were washed three times for 10 min and blocked with blocking solution 5% normal goat serum (NGS) for 1 h and incubated with 4-HNE primary antibody (#ab48506, mouse monoclonal, 1:25; Abcam, RRID:AB_867452) or TRPA1 (#ACC-037, rabbit polyclonal, 1:200; Alomone, RRID:AB_2040232; #NB110-40763, rabbit polyclonal, 1:200; Novus Biologicals, RRID:AB_715124) diluted in blocking solution for 1 h and washed two times for 10 min. .. Sections were then incubated with the fluorescent secondary antibodies Alexa Fluor® 594 (#A11005, goat polyclonal anti-mouse, 1:600; Thermo Fisher Scientific, RRID:AB_2534073), Alexa Fluor® 488 (#A32731, goat polyclonal anti-rabbit, 1:600; Thermo Fisher Scientific, RRID:AB_2633280) and Alexa Fluor® 594 (#A21207, donkey polyclonal anti-rabbit, Thermo Fisher Scientific, RRID:AB_ 141637) diluted in blocking solution for 2 h. After incubation with secondary antibody, the slides were washed two times for 10 min and slides containing 4-HNE antibody were coverslipped using the mounting medium with DAPI (#ab104139, Abcam); slides containing TRPA1 were incubated with S100 beta antibody (#ab196175, monoclonal anti-rabbit Alexa Fluor® 647, 1:50; Abcam, RRID:AB_ 2868562) or NeuN (#MAB377X, monoclonal anti-mouse Alexa Fluor® 488, 1:250; Merck, RRID:AB_2149209).

    Next-Generation Sequencing:

    Article Title: Schwann cell transient receptor potential ankyrin 1 (TRPA1) ortholog in zebrafish larvae mediates chemotherapy-induced peripheral neuropathy.
    Article Snippet: .. The slides were washed three times for 10 min and blocked with blocking solution 5% normal goat serum (NGS) for 1 h and incubated with 4-HNE primary antibody (#ab48506, mouse monoclonal, 1:25; Abcam, RRID:AB_867452) or TRPA1 (#ACC-037, rabbit polyclonal, 1:200; Alomone, RRID:AB_2040232; #NB110-40763, rabbit polyclonal, 1:200; Novus Biologicals, RRID:AB_715124) diluted in blocking solution for 1 h and washed two times for 10 min. .. Sections were then incubated with the fluorescent secondary antibodies Alexa Fluor® 594 (#A11005, goat polyclonal anti-mouse, 1:600; Thermo Fisher Scientific, RRID:AB_2534073), Alexa Fluor® 488 (#A32731, goat polyclonal anti-rabbit, 1:600; Thermo Fisher Scientific, RRID:AB_2633280) and Alexa Fluor® 594 (#A21207, donkey polyclonal anti-rabbit, Thermo Fisher Scientific, RRID:AB_ 141637) diluted in blocking solution for 2 h. After incubation with secondary antibody, the slides were washed two times for 10 min and slides containing 4-HNE antibody were coverslipped using the mounting medium with DAPI (#ab104139, Abcam); slides containing TRPA1 were incubated with S100 beta antibody (#ab196175, monoclonal anti-rabbit Alexa Fluor® 647, 1:50; Abcam, RRID:AB_ 2868562) or NeuN (#MAB377X, monoclonal anti-mouse Alexa Fluor® 488, 1:250; Merck, RRID:AB_2149209).

    Incubation:

    Article Title: Schwann cell transient receptor potential ankyrin 1 (TRPA1) ortholog in zebrafish larvae mediates chemotherapy-induced peripheral neuropathy.
    Article Snippet: .. The slides were washed three times for 10 min and blocked with blocking solution 5% normal goat serum (NGS) for 1 h and incubated with 4-HNE primary antibody (#ab48506, mouse monoclonal, 1:25; Abcam, RRID:AB_867452) or TRPA1 (#ACC-037, rabbit polyclonal, 1:200; Alomone, RRID:AB_2040232; #NB110-40763, rabbit polyclonal, 1:200; Novus Biologicals, RRID:AB_715124) diluted in blocking solution for 1 h and washed two times for 10 min. .. Sections were then incubated with the fluorescent secondary antibodies Alexa Fluor® 594 (#A11005, goat polyclonal anti-mouse, 1:600; Thermo Fisher Scientific, RRID:AB_2534073), Alexa Fluor® 488 (#A32731, goat polyclonal anti-rabbit, 1:600; Thermo Fisher Scientific, RRID:AB_2633280) and Alexa Fluor® 594 (#A21207, donkey polyclonal anti-rabbit, Thermo Fisher Scientific, RRID:AB_ 141637) diluted in blocking solution for 2 h. After incubation with secondary antibody, the slides were washed two times for 10 min and slides containing 4-HNE antibody were coverslipped using the mounting medium with DAPI (#ab104139, Abcam); slides containing TRPA1 were incubated with S100 beta antibody (#ab196175, monoclonal anti-rabbit Alexa Fluor® 647, 1:50; Abcam, RRID:AB_ 2868562) or NeuN (#MAB377X, monoclonal anti-mouse Alexa Fluor® 488, 1:250; Merck, RRID:AB_2149209).

    Inhibition:

    Article Title: Repetitive pulsed-wave ultrasound stimulation suppresses neural activity by modulating ambient GABA levels via effects on astrocytes
    Article Snippet: For activation of TRPA1, we used AITC ( ) (100 μM in DMSO, allyl isothiocyanate; Nacalai # 01415–92). .. For inhibition of TRPA1, we used HC-030031 ( ) (40 μM; Alomone # H-105). ..



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    Calcium imaging and immunocytochemistry showing TRPV1 expression in transfected HEK‐293 cells. (A) Fura2 calcium imaging in HEK‐293 cells transiently expressing rTRPV1‐EYFP fusion protein. Representative traces of calcium transients evoked by capsaicin in EYFP + cells. Green traces correspond to EYFP + cells and gray traces to EYFP − cells. Pie chart represents the percentage of EYFP + cells that responded or failded to respond to capsaicin. Data obtained from 88 cells from 3 coverslips. (B–F left) Confocal images of TRPV1‐EYFP transiently expressed in HEK‐293 cells. EYFP (green), TRPV1 antibody (magenta) and Hoechst (HO, blue). Merge images correspond to the overlap between TRPV1 and EYFP on the left and Hoechst and brightfield (BF) on the right. Antibody dilution of the representative images is indicated in the lower right corner. Scale bar: 20 μm. (B–F right) Box plots display the specificity ratio (SR) for each tested dilution of the corresponding antibody. Each dot represents a single EYFP + cell. Boxes indicate the interquartile range (25th to 75th percentiles) and whiskers extend to the 5th and 95th percentiles. The horizontal line inside each box marks the median and the black dot indicates the mean. (*** p < 0.001 Mann–Whitney test). (G) Bar histogram graph summarizes the SR median difference between the tested antibody dilution and their controls lacking primary antibody (secondary antibody only; rabbit, goat or guinea pig) as calculated with the Hodges‐Lehman estimator. Error bars represent the 95% confidence interval. For each antibody and dilution, a minimum of 100 cells were analyzed across 4 fields from 2 independent transfections. $ Santa Cruz V1 antibody was generated against a <t>human</t> <t>TRPA1</t> peptide.
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    Calcium imaging and immunocytochemistry showing TRPV1 expression in transfected HEK‐293 cells. (A) Fura2 calcium imaging in HEK‐293 cells transiently expressing rTRPV1‐EYFP fusion protein. Representative traces of calcium transients evoked by capsaicin in EYFP + cells. Green traces correspond to EYFP + cells and gray traces to EYFP − cells. Pie chart represents the percentage of EYFP + cells that responded or failded to respond to capsaicin. Data obtained from 88 cells from 3 coverslips. (B–F left) Confocal images of TRPV1‐EYFP transiently expressed in HEK‐293 cells. EYFP (green), TRPV1 antibody (magenta) and Hoechst (HO, blue). Merge images correspond to the overlap between TRPV1 and EYFP on the left and Hoechst and brightfield (BF) on the right. Antibody dilution of the representative images is indicated in the lower right corner. Scale bar: 20 μm. (B–F right) Box plots display the specificity ratio (SR) for each tested dilution of the corresponding antibody. Each dot represents a single EYFP + cell. Boxes indicate the interquartile range (25th to 75th percentiles) and whiskers extend to the 5th and 95th percentiles. The horizontal line inside each box marks the median and the black dot indicates the mean. (*** p < 0.001 Mann–Whitney test). (G) Bar histogram graph summarizes the SR median difference between the tested antibody dilution and their controls lacking primary antibody (secondary antibody only; rabbit, goat or guinea pig) as calculated with the Hodges‐Lehman estimator. Error bars represent the 95% confidence interval. For each antibody and dilution, a minimum of 100 cells were analyzed across 4 fields from 2 independent transfections. $ Santa Cruz V1 antibody was generated against a human <t>TRPA1</t> peptide.
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    Calcium imaging and immunocytochemistry showing TRPV1 expression in transfected HEK‐293 cells. (A) Fura2 calcium imaging in HEK‐293 cells transiently expressing rTRPV1‐EYFP fusion protein. Representative traces of calcium transients evoked by capsaicin in EYFP + cells. Green traces correspond to EYFP + cells and gray traces to EYFP − cells. Pie chart represents the percentage of EYFP + cells that responded or failded to respond to capsaicin. Data obtained from 88 cells from 3 coverslips. (B–F left) Confocal images of TRPV1‐EYFP transiently expressed in HEK‐293 cells. EYFP (green), TRPV1 antibody (magenta) and Hoechst (HO, blue). Merge images correspond to the overlap between TRPV1 and EYFP on the left and Hoechst and brightfield (BF) on the right. Antibody dilution of the representative images is indicated in the lower right corner. Scale bar: 20 μm. (B–F right) Box plots display the specificity ratio (SR) for each tested dilution of the corresponding antibody. Each dot represents a single EYFP + cell. Boxes indicate the interquartile range (25th to 75th percentiles) and whiskers extend to the 5th and 95th percentiles. The horizontal line inside each box marks the median and the black dot indicates the mean. (*** p < 0.001 Mann–Whitney test). (G) Bar histogram graph summarizes the SR median difference between the tested antibody dilution and their controls lacking primary antibody (secondary antibody only; rabbit, goat or guinea pig) as calculated with the Hodges‐Lehman estimator. Error bars represent the 95% confidence interval. For each antibody and dilution, a minimum of 100 cells were analyzed across 4 fields from 2 independent transfections. $ Santa Cruz V1 antibody was generated against a human <t>TRPA1</t> peptide.
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    Image Search Results


    Calcium imaging and immunocytochemistry showing TRPV1 expression in transfected HEK‐293 cells. (A) Fura2 calcium imaging in HEK‐293 cells transiently expressing rTRPV1‐EYFP fusion protein. Representative traces of calcium transients evoked by capsaicin in EYFP + cells. Green traces correspond to EYFP + cells and gray traces to EYFP − cells. Pie chart represents the percentage of EYFP + cells that responded or failded to respond to capsaicin. Data obtained from 88 cells from 3 coverslips. (B–F left) Confocal images of TRPV1‐EYFP transiently expressed in HEK‐293 cells. EYFP (green), TRPV1 antibody (magenta) and Hoechst (HO, blue). Merge images correspond to the overlap between TRPV1 and EYFP on the left and Hoechst and brightfield (BF) on the right. Antibody dilution of the representative images is indicated in the lower right corner. Scale bar: 20 μm. (B–F right) Box plots display the specificity ratio (SR) for each tested dilution of the corresponding antibody. Each dot represents a single EYFP + cell. Boxes indicate the interquartile range (25th to 75th percentiles) and whiskers extend to the 5th and 95th percentiles. The horizontal line inside each box marks the median and the black dot indicates the mean. (*** p < 0.001 Mann–Whitney test). (G) Bar histogram graph summarizes the SR median difference between the tested antibody dilution and their controls lacking primary antibody (secondary antibody only; rabbit, goat or guinea pig) as calculated with the Hodges‐Lehman estimator. Error bars represent the 95% confidence interval. For each antibody and dilution, a minimum of 100 cells were analyzed across 4 fields from 2 independent transfections. $ Santa Cruz V1 antibody was generated against a human TRPA1 peptide.

    Journal: Journal of Neurochemistry

    Article Title: Validation of TRPA1 and TRPV1 Antibodies for Expression Detection in Mammalian Cells and Tissues

    doi: 10.1111/jnc.70444

    Figure Lengend Snippet: Calcium imaging and immunocytochemistry showing TRPV1 expression in transfected HEK‐293 cells. (A) Fura2 calcium imaging in HEK‐293 cells transiently expressing rTRPV1‐EYFP fusion protein. Representative traces of calcium transients evoked by capsaicin in EYFP + cells. Green traces correspond to EYFP + cells and gray traces to EYFP − cells. Pie chart represents the percentage of EYFP + cells that responded or failded to respond to capsaicin. Data obtained from 88 cells from 3 coverslips. (B–F left) Confocal images of TRPV1‐EYFP transiently expressed in HEK‐293 cells. EYFP (green), TRPV1 antibody (magenta) and Hoechst (HO, blue). Merge images correspond to the overlap between TRPV1 and EYFP on the left and Hoechst and brightfield (BF) on the right. Antibody dilution of the representative images is indicated in the lower right corner. Scale bar: 20 μm. (B–F right) Box plots display the specificity ratio (SR) for each tested dilution of the corresponding antibody. Each dot represents a single EYFP + cell. Boxes indicate the interquartile range (25th to 75th percentiles) and whiskers extend to the 5th and 95th percentiles. The horizontal line inside each box marks the median and the black dot indicates the mean. (*** p < 0.001 Mann–Whitney test). (G) Bar histogram graph summarizes the SR median difference between the tested antibody dilution and their controls lacking primary antibody (secondary antibody only; rabbit, goat or guinea pig) as calculated with the Hodges‐Lehman estimator. Error bars represent the 95% confidence interval. For each antibody and dilution, a minimum of 100 cells were analyzed across 4 fields from 2 independent transfections. $ Santa Cruz V1 antibody was generated against a human TRPA1 peptide.

    Article Snippet: Alomone , TRPA1 , Rabbit , Alomone Labs , Human TRPA1 747–760. PC , 69.2% , ACC‐037 RRID:AB_2040232.

    Techniques: Imaging, Immunocytochemistry, Expressing, Transfection, MANN-WHITNEY, Generated

    Calcium imaging and immunocytochemistry of TRPA1 expression in transfected HEK‐ 293 cells. (A) Representative traces of Fura2 calcium transients evoked by 50 μM AITC. Green traces correspond to tGFP + cells and gray traces to tGFP − cells. The pie chart represents the proportion of tGFP + cells that responded or failed to respond to AITC. Data obtained from 115 cells from 3 coverslips. (B–H left) Confocal images of TRPA1‐tGFP transiently expressed in HEK‐ 293 cells incubated with the indicated antibody. tGFP (green), TRPA1 (magenta) and Hoechst staining (blue). Merge images correspond to the overlap between TRPA1 and tGFP (lower‐left panel) or Hoechst and brightfield (HO, BF, lower‐right). Antibody dilution of the representative images is indicated in the lower right‐hand corner. Scale bar: 20 μm. (B–H right) box plots display the specificity ratio (SR) for each tested dilution of the corresponding antibody. Each dot represents a single tGFP + cell. Boxes indicate the interquartile range (25th to 75th percentiles) and whiskers extend to the 5th and 95th percentiles. The horizontal line inside each box marks the median and the black dot indicates the mean. (** p < 0.01, *** p < 0.001 Mann–Whitney test). (I) Bar histogram graph summarizes the SR median difference between the tested antibody dilution and their controls lacking primary antibody (secondary antibody only; rabbit or mouse) as calculated with the Hodges‐Lehman estimator. Error bars represent the 95% confidence interval. For each antibody and dilution, a minimum of 319 cells were analyzed across 4 fields from 2 independent transfections.

    Journal: Journal of Neurochemistry

    Article Title: Validation of TRPA1 and TRPV1 Antibodies for Expression Detection in Mammalian Cells and Tissues

    doi: 10.1111/jnc.70444

    Figure Lengend Snippet: Calcium imaging and immunocytochemistry of TRPA1 expression in transfected HEK‐ 293 cells. (A) Representative traces of Fura2 calcium transients evoked by 50 μM AITC. Green traces correspond to tGFP + cells and gray traces to tGFP − cells. The pie chart represents the proportion of tGFP + cells that responded or failed to respond to AITC. Data obtained from 115 cells from 3 coverslips. (B–H left) Confocal images of TRPA1‐tGFP transiently expressed in HEK‐ 293 cells incubated with the indicated antibody. tGFP (green), TRPA1 (magenta) and Hoechst staining (blue). Merge images correspond to the overlap between TRPA1 and tGFP (lower‐left panel) or Hoechst and brightfield (HO, BF, lower‐right). Antibody dilution of the representative images is indicated in the lower right‐hand corner. Scale bar: 20 μm. (B–H right) box plots display the specificity ratio (SR) for each tested dilution of the corresponding antibody. Each dot represents a single tGFP + cell. Boxes indicate the interquartile range (25th to 75th percentiles) and whiskers extend to the 5th and 95th percentiles. The horizontal line inside each box marks the median and the black dot indicates the mean. (** p < 0.01, *** p < 0.001 Mann–Whitney test). (I) Bar histogram graph summarizes the SR median difference between the tested antibody dilution and their controls lacking primary antibody (secondary antibody only; rabbit or mouse) as calculated with the Hodges‐Lehman estimator. Error bars represent the 95% confidence interval. For each antibody and dilution, a minimum of 319 cells were analyzed across 4 fields from 2 independent transfections.

    Article Snippet: Alomone , TRPA1 , Rabbit , Alomone Labs , Human TRPA1 747–760. PC , 69.2% , ACC‐037 RRID:AB_2040232.

    Techniques: Imaging, Immunocytochemistry, Expressing, Transfection, Incubation, Staining, MANN-WHITNEY

    Western blot analysis for TRPV1 and TRPA1 antibodies specificity. (A) Immunoblots for TRPV1 using Abcam (1:1000), Millipore V1 (1:1000), Santa Cruz V1 (1:100), Neuromics GT (1:200), and Neuromics GP (1:1000) antibodies. (−) lanes: Untransfected HEK‐293 cells, (+) lanes: HEK‐293 cells transfected with rTRPV1‐EYFP (B) Immunoblots for TRPA1 using Aviva (1:300), Novus (1:500), Millipore A1 (1:500), Alomone (1:200), Proteintech (1:500), Sigma WH (1:500), and Santa Cruz A1 (1:100). (−) lanes: Untransfected HEK‐293 cells, (+) lanes: HEK‐293 cells transfected with hTRPA1‐tGFFP. For each antibody, top row: Immunoblot with each TRPV1/TRPA1 antibody. Middle row: EYFP/tGFP immunoblotting of the same membrane. Bottom image: GAPDH loading control. Each blot was performed at least three times to rule out technical artifacts in cases where no anti‐TRPV1/TRPA1 signal was detected. For every repetition, the identical lysate sample was probed with all antibodies. Full uncropped blots are available in the Figure . $ Santa Cruz V1 antibody was generated against a human TRPA1 peptide.

    Journal: Journal of Neurochemistry

    Article Title: Validation of TRPA1 and TRPV1 Antibodies for Expression Detection in Mammalian Cells and Tissues

    doi: 10.1111/jnc.70444

    Figure Lengend Snippet: Western blot analysis for TRPV1 and TRPA1 antibodies specificity. (A) Immunoblots for TRPV1 using Abcam (1:1000), Millipore V1 (1:1000), Santa Cruz V1 (1:100), Neuromics GT (1:200), and Neuromics GP (1:1000) antibodies. (−) lanes: Untransfected HEK‐293 cells, (+) lanes: HEK‐293 cells transfected with rTRPV1‐EYFP (B) Immunoblots for TRPA1 using Aviva (1:300), Novus (1:500), Millipore A1 (1:500), Alomone (1:200), Proteintech (1:500), Sigma WH (1:500), and Santa Cruz A1 (1:100). (−) lanes: Untransfected HEK‐293 cells, (+) lanes: HEK‐293 cells transfected with hTRPA1‐tGFFP. For each antibody, top row: Immunoblot with each TRPV1/TRPA1 antibody. Middle row: EYFP/tGFP immunoblotting of the same membrane. Bottom image: GAPDH loading control. Each blot was performed at least three times to rule out technical artifacts in cases where no anti‐TRPV1/TRPA1 signal was detected. For every repetition, the identical lysate sample was probed with all antibodies. Full uncropped blots are available in the Figure . $ Santa Cruz V1 antibody was generated against a human TRPA1 peptide.

    Article Snippet: Alomone , TRPA1 , Rabbit , Alomone Labs , Human TRPA1 747–760. PC , 69.2% , ACC‐037 RRID:AB_2040232.

    Techniques: Western Blot, Transfection, Membrane, Control, Generated

    Calcium imaging and Immunochemistry of endogenous TRPA1 in cultured DRG neurons from TRPA1‐Cre‐ChR2‐EYFP mice. (A) Representative traces of calcium transients evoked by AITC and capsaicin in DRG neurons. Traces corresponding to cells responding to 50 μM AITC are displayed in yellow and non‐responding cells are displayed in gray. (B) The pie chart represents the proportion of neurons responding or failing to respond to AITC. Data obtained from 424 cells from 5 coverslips. (C–F) ICC or (G–J) IHC confocal images of DRG sensory neurons incubated with the indicated antibody. TRPA1 antibody (magenta) and βIIITubulin (cyan), merge images display TRPA1 antibody signal + βIII‐Tubulin. Dilution of the corresponding antibody shown in each example is indicated in the lower right corner. Scale bar: 50 μm. Representative images were chosen from 4 pictures of 2 different animals.

    Journal: Journal of Neurochemistry

    Article Title: Validation of TRPA1 and TRPV1 Antibodies for Expression Detection in Mammalian Cells and Tissues

    doi: 10.1111/jnc.70444

    Figure Lengend Snippet: Calcium imaging and Immunochemistry of endogenous TRPA1 in cultured DRG neurons from TRPA1‐Cre‐ChR2‐EYFP mice. (A) Representative traces of calcium transients evoked by AITC and capsaicin in DRG neurons. Traces corresponding to cells responding to 50 μM AITC are displayed in yellow and non‐responding cells are displayed in gray. (B) The pie chart represents the proportion of neurons responding or failing to respond to AITC. Data obtained from 424 cells from 5 coverslips. (C–F) ICC or (G–J) IHC confocal images of DRG sensory neurons incubated with the indicated antibody. TRPA1 antibody (magenta) and βIIITubulin (cyan), merge images display TRPA1 antibody signal + βIII‐Tubulin. Dilution of the corresponding antibody shown in each example is indicated in the lower right corner. Scale bar: 50 μm. Representative images were chosen from 4 pictures of 2 different animals.

    Article Snippet: Alomone , TRPA1 , Rabbit , Alomone Labs , Human TRPA1 747–760. PC , 69.2% , ACC‐037 RRID:AB_2040232.

    Techniques: Imaging, Cell Culture, Incubation

    Calcium imaging and immunocytochemistry showing TRPV1 expression in transfected HEK‐293 cells. (A) Fura2 calcium imaging in HEK‐293 cells transiently expressing rTRPV1‐EYFP fusion protein. Representative traces of calcium transients evoked by capsaicin in EYFP + cells. Green traces correspond to EYFP + cells and gray traces to EYFP − cells. Pie chart represents the percentage of EYFP + cells that responded or failded to respond to capsaicin. Data obtained from 88 cells from 3 coverslips. (B–F left) Confocal images of TRPV1‐EYFP transiently expressed in HEK‐293 cells. EYFP (green), TRPV1 antibody (magenta) and Hoechst (HO, blue). Merge images correspond to the overlap between TRPV1 and EYFP on the left and Hoechst and brightfield (BF) on the right. Antibody dilution of the representative images is indicated in the lower right corner. Scale bar: 20 μm. (B–F right) Box plots display the specificity ratio (SR) for each tested dilution of the corresponding antibody. Each dot represents a single EYFP + cell. Boxes indicate the interquartile range (25th to 75th percentiles) and whiskers extend to the 5th and 95th percentiles. The horizontal line inside each box marks the median and the black dot indicates the mean. (*** p < 0.001 Mann–Whitney test). (G) Bar histogram graph summarizes the SR median difference between the tested antibody dilution and their controls lacking primary antibody (secondary antibody only; rabbit, goat or guinea pig) as calculated with the Hodges‐Lehman estimator. Error bars represent the 95% confidence interval. For each antibody and dilution, a minimum of 100 cells were analyzed across 4 fields from 2 independent transfections. $ Santa Cruz V1 antibody was generated against a human TRPA1 peptide.

    Journal: Journal of Neurochemistry

    Article Title: Validation of TRPA1 and TRPV1 Antibodies for Expression Detection in Mammalian Cells and Tissues

    doi: 10.1111/jnc.70444

    Figure Lengend Snippet: Calcium imaging and immunocytochemistry showing TRPV1 expression in transfected HEK‐293 cells. (A) Fura2 calcium imaging in HEK‐293 cells transiently expressing rTRPV1‐EYFP fusion protein. Representative traces of calcium transients evoked by capsaicin in EYFP + cells. Green traces correspond to EYFP + cells and gray traces to EYFP − cells. Pie chart represents the percentage of EYFP + cells that responded or failded to respond to capsaicin. Data obtained from 88 cells from 3 coverslips. (B–F left) Confocal images of TRPV1‐EYFP transiently expressed in HEK‐293 cells. EYFP (green), TRPV1 antibody (magenta) and Hoechst (HO, blue). Merge images correspond to the overlap between TRPV1 and EYFP on the left and Hoechst and brightfield (BF) on the right. Antibody dilution of the representative images is indicated in the lower right corner. Scale bar: 20 μm. (B–F right) Box plots display the specificity ratio (SR) for each tested dilution of the corresponding antibody. Each dot represents a single EYFP + cell. Boxes indicate the interquartile range (25th to 75th percentiles) and whiskers extend to the 5th and 95th percentiles. The horizontal line inside each box marks the median and the black dot indicates the mean. (*** p < 0.001 Mann–Whitney test). (G) Bar histogram graph summarizes the SR median difference between the tested antibody dilution and their controls lacking primary antibody (secondary antibody only; rabbit, goat or guinea pig) as calculated with the Hodges‐Lehman estimator. Error bars represent the 95% confidence interval. For each antibody and dilution, a minimum of 100 cells were analyzed across 4 fields from 2 independent transfections. $ Santa Cruz V1 antibody was generated against a human TRPA1 peptide.

    Article Snippet: Novus , TRPA1 , Rabbit , Novus Biologicals (Biotechne) , Human TRPA1 N‐terminus (1–100) PC , 77% , NB110‐40763 RRID:AB_715124.

    Techniques: Imaging, Immunocytochemistry, Expressing, Transfection, MANN-WHITNEY, Generated

    Calcium imaging and immunocytochemistry of TRPA1 expression in transfected HEK‐ 293 cells. (A) Representative traces of Fura2 calcium transients evoked by 50 μM AITC. Green traces correspond to tGFP + cells and gray traces to tGFP − cells. The pie chart represents the proportion of tGFP + cells that responded or failed to respond to AITC. Data obtained from 115 cells from 3 coverslips. (B–H left) Confocal images of TRPA1‐tGFP transiently expressed in HEK‐ 293 cells incubated with the indicated antibody. tGFP (green), TRPA1 (magenta) and Hoechst staining (blue). Merge images correspond to the overlap between TRPA1 and tGFP (lower‐left panel) or Hoechst and brightfield (HO, BF, lower‐right). Antibody dilution of the representative images is indicated in the lower right‐hand corner. Scale bar: 20 μm. (B–H right) box plots display the specificity ratio (SR) for each tested dilution of the corresponding antibody. Each dot represents a single tGFP + cell. Boxes indicate the interquartile range (25th to 75th percentiles) and whiskers extend to the 5th and 95th percentiles. The horizontal line inside each box marks the median and the black dot indicates the mean. (** p < 0.01, *** p < 0.001 Mann–Whitney test). (I) Bar histogram graph summarizes the SR median difference between the tested antibody dilution and their controls lacking primary antibody (secondary antibody only; rabbit or mouse) as calculated with the Hodges‐Lehman estimator. Error bars represent the 95% confidence interval. For each antibody and dilution, a minimum of 319 cells were analyzed across 4 fields from 2 independent transfections.

    Journal: Journal of Neurochemistry

    Article Title: Validation of TRPA1 and TRPV1 Antibodies for Expression Detection in Mammalian Cells and Tissues

    doi: 10.1111/jnc.70444

    Figure Lengend Snippet: Calcium imaging and immunocytochemistry of TRPA1 expression in transfected HEK‐ 293 cells. (A) Representative traces of Fura2 calcium transients evoked by 50 μM AITC. Green traces correspond to tGFP + cells and gray traces to tGFP − cells. The pie chart represents the proportion of tGFP + cells that responded or failed to respond to AITC. Data obtained from 115 cells from 3 coverslips. (B–H left) Confocal images of TRPA1‐tGFP transiently expressed in HEK‐ 293 cells incubated with the indicated antibody. tGFP (green), TRPA1 (magenta) and Hoechst staining (blue). Merge images correspond to the overlap between TRPA1 and tGFP (lower‐left panel) or Hoechst and brightfield (HO, BF, lower‐right). Antibody dilution of the representative images is indicated in the lower right‐hand corner. Scale bar: 20 μm. (B–H right) box plots display the specificity ratio (SR) for each tested dilution of the corresponding antibody. Each dot represents a single tGFP + cell. Boxes indicate the interquartile range (25th to 75th percentiles) and whiskers extend to the 5th and 95th percentiles. The horizontal line inside each box marks the median and the black dot indicates the mean. (** p < 0.01, *** p < 0.001 Mann–Whitney test). (I) Bar histogram graph summarizes the SR median difference between the tested antibody dilution and their controls lacking primary antibody (secondary antibody only; rabbit or mouse) as calculated with the Hodges‐Lehman estimator. Error bars represent the 95% confidence interval. For each antibody and dilution, a minimum of 319 cells were analyzed across 4 fields from 2 independent transfections.

    Article Snippet: Novus , TRPA1 , Rabbit , Novus Biologicals (Biotechne) , Human TRPA1 N‐terminus (1–100) PC , 77% , NB110‐40763 RRID:AB_715124.

    Techniques: Imaging, Immunocytochemistry, Expressing, Transfection, Incubation, Staining, MANN-WHITNEY

    Western blot analysis for TRPV1 and TRPA1 antibodies specificity. (A) Immunoblots for TRPV1 using Abcam (1:1000), Millipore V1 (1:1000), Santa Cruz V1 (1:100), Neuromics GT (1:200), and Neuromics GP (1:1000) antibodies. (−) lanes: Untransfected HEK‐293 cells, (+) lanes: HEK‐293 cells transfected with rTRPV1‐EYFP (B) Immunoblots for TRPA1 using Aviva (1:300), Novus (1:500), Millipore A1 (1:500), Alomone (1:200), Proteintech (1:500), Sigma WH (1:500), and Santa Cruz A1 (1:100). (−) lanes: Untransfected HEK‐293 cells, (+) lanes: HEK‐293 cells transfected with hTRPA1‐tGFFP. For each antibody, top row: Immunoblot with each TRPV1/TRPA1 antibody. Middle row: EYFP/tGFP immunoblotting of the same membrane. Bottom image: GAPDH loading control. Each blot was performed at least three times to rule out technical artifacts in cases where no anti‐TRPV1/TRPA1 signal was detected. For every repetition, the identical lysate sample was probed with all antibodies. Full uncropped blots are available in the Figure . $ Santa Cruz V1 antibody was generated against a human TRPA1 peptide.

    Journal: Journal of Neurochemistry

    Article Title: Validation of TRPA1 and TRPV1 Antibodies for Expression Detection in Mammalian Cells and Tissues

    doi: 10.1111/jnc.70444

    Figure Lengend Snippet: Western blot analysis for TRPV1 and TRPA1 antibodies specificity. (A) Immunoblots for TRPV1 using Abcam (1:1000), Millipore V1 (1:1000), Santa Cruz V1 (1:100), Neuromics GT (1:200), and Neuromics GP (1:1000) antibodies. (−) lanes: Untransfected HEK‐293 cells, (+) lanes: HEK‐293 cells transfected with rTRPV1‐EYFP (B) Immunoblots for TRPA1 using Aviva (1:300), Novus (1:500), Millipore A1 (1:500), Alomone (1:200), Proteintech (1:500), Sigma WH (1:500), and Santa Cruz A1 (1:100). (−) lanes: Untransfected HEK‐293 cells, (+) lanes: HEK‐293 cells transfected with hTRPA1‐tGFFP. For each antibody, top row: Immunoblot with each TRPV1/TRPA1 antibody. Middle row: EYFP/tGFP immunoblotting of the same membrane. Bottom image: GAPDH loading control. Each blot was performed at least three times to rule out technical artifacts in cases where no anti‐TRPV1/TRPA1 signal was detected. For every repetition, the identical lysate sample was probed with all antibodies. Full uncropped blots are available in the Figure . $ Santa Cruz V1 antibody was generated against a human TRPA1 peptide.

    Article Snippet: Novus , TRPA1 , Rabbit , Novus Biologicals (Biotechne) , Human TRPA1 N‐terminus (1–100) PC , 77% , NB110‐40763 RRID:AB_715124.

    Techniques: Western Blot, Transfection, Membrane, Control, Generated

    Calcium imaging and Immunochemistry of endogenous TRPA1 in cultured DRG neurons from TRPA1‐Cre‐ChR2‐EYFP mice. (A) Representative traces of calcium transients evoked by AITC and capsaicin in DRG neurons. Traces corresponding to cells responding to 50 μM AITC are displayed in yellow and non‐responding cells are displayed in gray. (B) The pie chart represents the proportion of neurons responding or failing to respond to AITC. Data obtained from 424 cells from 5 coverslips. (C–F) ICC or (G–J) IHC confocal images of DRG sensory neurons incubated with the indicated antibody. TRPA1 antibody (magenta) and βIIITubulin (cyan), merge images display TRPA1 antibody signal + βIII‐Tubulin. Dilution of the corresponding antibody shown in each example is indicated in the lower right corner. Scale bar: 50 μm. Representative images were chosen from 4 pictures of 2 different animals.

    Journal: Journal of Neurochemistry

    Article Title: Validation of TRPA1 and TRPV1 Antibodies for Expression Detection in Mammalian Cells and Tissues

    doi: 10.1111/jnc.70444

    Figure Lengend Snippet: Calcium imaging and Immunochemistry of endogenous TRPA1 in cultured DRG neurons from TRPA1‐Cre‐ChR2‐EYFP mice. (A) Representative traces of calcium transients evoked by AITC and capsaicin in DRG neurons. Traces corresponding to cells responding to 50 μM AITC are displayed in yellow and non‐responding cells are displayed in gray. (B) The pie chart represents the proportion of neurons responding or failing to respond to AITC. Data obtained from 424 cells from 5 coverslips. (C–F) ICC or (G–J) IHC confocal images of DRG sensory neurons incubated with the indicated antibody. TRPA1 antibody (magenta) and βIIITubulin (cyan), merge images display TRPA1 antibody signal + βIII‐Tubulin. Dilution of the corresponding antibody shown in each example is indicated in the lower right corner. Scale bar: 50 μm. Representative images were chosen from 4 pictures of 2 different animals.

    Article Snippet: Novus , TRPA1 , Rabbit , Novus Biologicals (Biotechne) , Human TRPA1 N‐terminus (1–100) PC , 77% , NB110‐40763 RRID:AB_715124.

    Techniques: Imaging, Cell Culture, Incubation