anti trpa1 (Alomone Labs)
Structured Review
Anti Trpa1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trpa1/Anti-TRPA1+(extracellular)+Antibody/bio_rxiv__64898__2026__05__11__724406-42-19-23
Average 95 stars, based on 55 article reviews
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Western Blot:Article Title: Schwann cell Lrp1 deletion drives trigeminal neuron sensitization and orofacial pain by modulating mitochondrial function and TRPV1/TRPA1 activity. Article Snippet: .. Primary antibodies and dilutions: MFN2 (Proteintech, #12186–1-AP, Rabbit, 1:1000); Phospho-PDH (pPDH) (Cell Signaling Technology, #31866, Rabbit, 1:1000); Total PDH (tPDH) (Cell Signaling Technology, #3205, Rabbit, 1:1000); Total OXPHOS Rodent WB Antibody Cocktail (Abcam, ab110413, Mouse, 1:1000); TRPV1 (Alomone Labs, Israel, ACC-030, Rabbit, 1:500); TRPA1 (Alomone Labs, Israel, ACC-037, Rabbit, 1:500). .. Secondary antibodies: Anti-Rabbit IgG, HRP-linked (Cell Signaling Technology, #7074, 1:3000); Anti-Mouse IgG, HRP-linked (Cell Signaling Technology, #7076, 1:3000). Article Title: Schwann cell Lrp1 deletion drives trigeminal neuron sensitization and orofacial pain by modulating mitochondrial function and TRPV1/TRPA1 activity Article Snippet: .. Primary antibodies and dilutions: MFN2 (Proteintech, #12186–1-AP, Rabbit, 1:1000); Phospho-PDH (pPDH) (Cell Signaling Technology, #31866, Rabbit, 1:1000); Total PDH (tPDH) (Cell Signaling Technology, #3205, Rabbit, 1:1000); Total OXPHOS Rodent WB Antibody Cocktail (Abcam, ab110413, Mouse, 1:1000); TRPV1 (Alomone Labs, Israel, ACC-030, Rabbit, 1:500); TRPA1 (Alomone Labs, Israel, ACC-037, Rabbit, 1:500). .. Secondary antibodies: Anti-Rabbit IgG, HRP-linked (Cell Signaling Technology, #7074, 1:3000); Anti-Mouse IgG, HRP-linked (Cell Signaling Technology, #7076, 1:3000). Activation Assay:Article Title: TRPA1 contributes to respiratory depression from tobacco aerosol. Article Snippet: Transient receptor potential ankyrin-1 (TRPA1) is expressed in the trigeminal nerves in the nasal cavity.. It detects irritant chemicals such as formalin and acrolein, induces respiratory depression to protect against further inhalation, and elicits avoidance behavior.. Although tobacco smoke contains formalin, acrolein, and other irritant chemicals, the possible contribution of TRPA1 to protection against tobacco smoke has yet to be fully understood. Aerosol:Article Title: TRPA1 contributes to respiratory depression from tobacco aerosol. Article Snippet: Transient receptor potential ankyrin-1 (TRPA1) is expressed in the trigeminal nerves in the nasal cavity.. It detects irritant chemicals such as formalin and acrolein, induces respiratory depression to protect against further inhalation, and elicits avoidance behavior.. Although tobacco smoke contains formalin, acrolein, and other irritant chemicals, the possible contribution of TRPA1 to protection against tobacco smoke has yet to be fully understood. Staining:Article Title: TRPA1 contributes to respiratory depression from tobacco aerosol. Article Snippet: Transient receptor potential ankyrin-1 (TRPA1) is expressed in the trigeminal nerves in the nasal cavity.. It detects irritant chemicals such as formalin and acrolein, induces respiratory depression to protect against further inhalation, and elicits avoidance behavior.. Although tobacco smoke contains formalin, acrolein, and other irritant chemicals, the possible contribution of TRPA1 to protection against tobacco smoke has yet to be fully understood. Article Title: Few-layered graphene increases the response of nociceptive neurons to irritant stimuli. Article Snippet: The fluorescently conjugated secondary antibodies were labeled by Alexa Fluor 488 or Alexa Fluor 568 (#11029 and #A11036, respectively; ThermoFisher Scientific, Molecular Probes). .. Finally, after another washing round, neurons were stained with Hoechst33342 diluted 1 : 100 in BSA (2%) for 5 min. For studies on the channel distribution, polyclonal antiTRPV1 (ACC-334, Alomone labs) and Immunohistochemistry:Article Title: Harnessing theta-gamma coupled brainwaves using ultrasound for spinal astrocyte revitalization and sustained neuropathic pain relief in mice Article Snippet: The tissue was then sectioned at 30 μm into cold PBS using a vibratome (Leica VT1000S) and stored at 4 °C. .. For spinal cord immunohistochemistry, spinal sections were immunolabeled for GFAP, GABA, BDNF, Iba1 (1:1000, ab225260), NeuN (1:1000, Millipore, MAB377), c-Fos (1:1000, SYSY, 226-008), and Article Title: Harnessing theta-gamma coupled brainwaves using ultrasound for spinal astrocyte revitalization and sustained neuropathic pain relief in mice. Article Snippet: The tissue was then sectioned at 30 μm into cold PBS using vibratome Leica VT1000S and stored at 4°C. .. For spinal cord immunohistochemistry, spinal sections were immunolabeled for GFAP, GABA, BDNF, Iba1 (1:1000, ab225260), NeuN (1:1000, Millipore, MAB377), c-Fos (1:1000, SYSY, 226-008), Immunolabeling:Article Title: Harnessing theta-gamma coupled brainwaves using ultrasound for spinal astrocyte revitalization and sustained neuropathic pain relief in mice Article Snippet: The tissue was then sectioned at 30 μm into cold PBS using a vibratome (Leica VT1000S) and stored at 4 °C. .. For spinal cord immunohistochemistry, spinal sections were immunolabeled for GFAP, GABA, BDNF, Iba1 (1:1000, ab225260), NeuN (1:1000, Millipore, MAB377), c-Fos (1:1000, SYSY, 226-008), and Article Title: Harnessing theta-gamma coupled brainwaves using ultrasound for spinal astrocyte revitalization and sustained neuropathic pain relief in mice. Article Snippet: The tissue was then sectioned at 30 μm into cold PBS using vibratome Leica VT1000S and stored at 4°C. .. For spinal cord immunohistochemistry, spinal sections were immunolabeled for GFAP, GABA, BDNF, Iba1 (1:1000, ab225260), NeuN (1:1000, Millipore, MAB377), c-Fos (1:1000, SYSY, 226-008), Blocking Assay:Article Title: Schwann cell transient receptor potential ankyrin 1 (TRPA1) ortholog in zebrafish larvae mediates chemotherapy-induced peripheral neuropathy. Article Snippet: .. The slides were washed three times for 10 min and blocked with blocking solution 5% normal goat serum (NGS) for 1 h and incubated with 4-HNE primary antibody (#ab48506, mouse monoclonal, 1:25; Abcam, RRID:AB_867452) or Next-Generation Sequencing:Article Title: Schwann cell transient receptor potential ankyrin 1 (TRPA1) ortholog in zebrafish larvae mediates chemotherapy-induced peripheral neuropathy. Article Snippet: .. The slides were washed three times for 10 min and blocked with blocking solution 5% normal goat serum (NGS) for 1 h and incubated with 4-HNE primary antibody (#ab48506, mouse monoclonal, 1:25; Abcam, RRID:AB_867452) or Incubation:Article Title: Schwann cell transient receptor potential ankyrin 1 (TRPA1) ortholog in zebrafish larvae mediates chemotherapy-induced peripheral neuropathy. Article Snippet: .. The slides were washed three times for 10 min and blocked with blocking solution 5% normal goat serum (NGS) for 1 h and incubated with 4-HNE primary antibody (#ab48506, mouse monoclonal, 1:25; Abcam, RRID:AB_867452) or Inhibition:Article Title: Repetitive pulsed-wave ultrasound stimulation suppresses neural activity by modulating ambient GABA levels via effects on astrocytes Article Snippet: For activation of TRPA1, we used AITC ( ) (100 μM in DMSO, allyl isothiocyanate; Nacalai # 01415–92). .. For inhibition of |
